The player, who tasted cup glory with the club by lifting the 2017/18 Nedbank Cup title was killed during the fatal accident in Bethlehem at approximately 6am on Monday morning.
Jantjie was set to leave the club at the end of this season after already having signed a pre-contract with Wits.
Ea Lla Koto confirmed that the funeral and memorial arrangements will be announced in due course.
The club confirmed the tragic news in a brief statement on their website.
"It is with great sadness that we announce the passing of Free State Stars midfielder Sinethemba Jantjie.
"Mr Jantjie passed away in the early hours of the morning when his car was involved in an accident.
"The club plans to meet with Jantjie’s family, after which they will give further details of the funeral and memorial arrangements.
Jantjie was a late bloomer in the PSL, making his debut in his late 20s in 2016/17. He was a regular starter for Stars and his good form caught the eye of Gavin Hunt at Bidvest Wits who had signed him on a pre-contract for next season."
Jantjie was 30-years-old.
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